New PDF release: Affinity Techniques - Enzyme Purification: Part B

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121818977

ISBN-13: 9780121818975

The severely acclaimed laboratory regular, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - really an important e-book for researchers in all fields of lifestyles sciences

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Model chromatogram illustrating biospecific affinity chromatography with one-step elution (displacement) using a nonspeciflc displacer. Striped areas represent impurities. [2] GENERAL METHODS AND COUPLING PROCEDURES 17 Concentration "///× ~4q ~//~ J ~/,/', .... J J~ J f// Strong displacer rinsing) Effluent volume FIG. 2. Model chromatogram illustrating fractionation and incomplete purification of specifically adsorbed substances by means of a concentration gradient of a nonspecific eluting agent ( - - - ) .

Cuatrecasas, Advan. Enzymol. 36, 29 (1972). =5j. K. Inman, unpublished work, 1973. [3] COVALENT LINKAGES TO POLYACRYLAMIDE 39 anhydride 2~,~4 ( V ) is added in an a m o u n t equal to 3 mmoles per millimole of hydrazide g r o u p estimated to be present. A m i n i m u m of 3 m g of anhydride should be added per milliliter of suspension. 3 by additions of 1 N (or 2 N ) N a O H . Reaction is complete in 1 5 - 2 0 minutes. A small sample of gel m a y be washed on a funnel with saline and given the T N B S test.

Truffa-Bachi and L. Wofsy, Proc. Nat. Acad. Sci. S. 66, 685, (1970). 7L. Wofsy, J. Kimura, and P. Truffa-Bachi, J. Immunol. 107, 725 (1971); C. Henry, J. Kimura, and L. Wofsy, Proc. Nat. Acad. Sci. S. 69, 34 (1972). 8 j. K. Inman, in "Mechanisms of Cell-Mediated Immunity" (R. T. McCluskey and S. ), p. 375. Wiley (Interscience), New York, 1974. ~S. Sell and T. An, J. lmmunol. 107, 1302 (1971); J. D. Stobo, A. S. Rosenthal, and W. E. Paul, J. lmmunol. 108, 1 (1972). ~ep. G. H. Gell and H. P. " Karger, Basel, in press.

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Affinity Techniques - Enzyme Purification: Part B by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

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